rg2 gbm cell lines  (ATCC)

Journal: Molecular Therapy

Article Title: Efficacy and Safety/Toxicity Study of Recombinant Vaccinia Virus JX-594 in Two Immunocompetent Animal Models of Glioma

doi: 10.1038/mt.2010.183

Figure Lengend Snippet: i.t. administration of JX-594/JX-594m inhibited tumor growth and prolonged survival of immunocompetent animals-bearing intracranial glioma . ( a ) Kaplan–Meier survival of rats harboring intracranial RG2 tumor treated with PBS ( n = 8) or i.t. administration of JX-594 ( n = 7, 5 × 10 7 PFUs /rat) or i.t. administration of JX-594m ( n = 8, 5 × 10 7 /rat, at days 1 and 4). Arrows indicates virus administration. ( b ) Representative BLI obtained at days 4, 11, and 14 after tumor implantation of RG2-Fluc and treatment with JX-594, JX-594m, or PBS. ( c ) Quantification of the BLI. ( d ) Kaplan–Meier survival curves of C57/BL6 mice harboring GL261 tumor treated with control (PBS, n = 7), JX-594 ( n = 7, 1 × 10 7 PFU/rat for three times, at days 1, 4, and 10) or JX-594m ( n = 8). Arrows indicate the day of virus administration. BLI, bioluminescence image; i.t., intracranial; PBS, phosphate-buffered saline; PFU, plaque-forming unit; p.i., postinfection.

Article Snippet: Murine NIH3T3 fibroblasts and racine GBM cell lines RG2, F98, and human U87, U118 are from the American Type Culture Collection (Manassas, VA).

Techniques: Virus, Tumor Implantation, Control, Saline

Journal: Molecular Therapy

Article Title: Efficacy and Safety/Toxicity Study of Recombinant Vaccinia Virus JX-594 in Two Immunocompetent Animal Models of Glioma

doi: 10.1038/mt.2010.183

Figure Lengend Snippet: Combination therapy with rapamycin promotes oncolysis in vitro and enhanced viral replication in vivo . ( a , b ) Viability of rodents glioma cell lines (RG2, GL261) were measured using the MTT assay 24 and 72 hours postinfection with JX-594 (JX), rapamycin alone (Rap), or both (JX+Rap). ** P < 0.05 as analyze by two-way ANOVA. ( c ) Representative viral replication BLI images (top) and quantification of BLI in JX-594Fluc alone ( n = 3) or JX-594Fluc + rapamycin ( n = 3) treated RG2 tumor-bearing rats (bottom). ( d ) Representative viral replication quantification of BLI in JX-594Fluc alone ( n = 3) or JX-594Fluc + rapamycin ( n = 3) treated GL261 tumor-bearing mice. BLI, bioluminescence image; p.i., postinfection.

Article Snippet: Murine NIH3T3 fibroblasts and racine GBM cell lines RG2, F98, and human U87, U118 are from the American Type Culture Collection (Manassas, VA).

Techniques: In Vitro, In Vivo, MTT Assay

Journal: Molecular Therapy

Article Title: Efficacy and Safety/Toxicity Study of Recombinant Vaccinia Virus JX-594 in Two Immunocompetent Animal Models of Glioma

doi: 10.1038/mt.2010.183

Figure Lengend Snippet: Combination therapy with rapamycin promotes JX-594/JX-594m oncolysis in vitro and in vivo . ( a ) Kaplan–Meier survival of intracranial RG2-bearing rats after treatment with PBS ( n = 4), JX-594 alone ( n = 6), rapamycin alone ( n = 4), or a combination of both JX-594 and rapamycin ( n = 6, log-rank test, P < 0.0001 compared to PBS). ( b ) Kaplan–Meier survival of “advanced” intracranial RG2 tumor-bearing rats after treatment with PBS ( n = 6), JX-594m alone ( n = 7), rapamycin alone ( n = 6), or a combination of both JX-594m and rapamycin ( n = 7, log-rank test, P < 0.0001 compared to PBS). ( c ) Survival of intracranial GL261 tumor-bearing mice after treatment with PBS ( n = 5), JX-594 alone ( n = 7), rapamycin alone ( n = 5), or a combination of both JX-594 and rapamycin ( n = 7, log-rank test, P < 0.0001 compared to PBS). ( d ) Kaplan–Meier survival of “advanced” intracranial GL261 tumor-bearing mice after treatment with PBS ( n = 6), JX-594m alone ( n = 6), rapamycin alone ( n = 6), or a combination of both JX-594m and rapamycin ( n = 7, log-rank test, P = 0.1524 compared to PBS). PBS, phosphate-buffered saline; Rap, rapamycin.

Article Snippet: Murine NIH3T3 fibroblasts and racine GBM cell lines RG2, F98, and human U87, U118 are from the American Type Culture Collection (Manassas, VA).

Techniques: In Vitro, In Vivo, Saline